1. Trihydroxymethyl aminomethane maka mmepụta njikọ ntụziaka

N'ihi nhazi ya dị mfe, etiti carbon atom bụ ihe owuwu tetrahedral, nke nwere hydroxymethyl atọ na otu amino, nke nwere ike ime mgbanwe dị iche iche na mmeghachi omume REDOX, bụ ụdị ihe nrụpụta kemịkalụ dị elu. Otu n'ime usoro njikọ nke ihe nchekwa TAPS mere site na iji Tris na 1, 3-propanolone na mmanya. Ọtụtụ mgbe, akụrụngwa TRIS ejiri maka njikọ ụlọ ọrụ anaghị achọ ịdị ọcha nke reagent dị elu. Ihe a na-emesi ike bụ nnukwu ọnụ ọgụgụ, ya mere ọ dị ọnụ ahịa karịa ịchọta onye na-emepụta ihe na-emepụta ihe na nnukwu ikike.

2. Trimethylol amino methane maka ntụziaka nchọpụta biochemical

A na-ejikarị Tris eme ihe dị ka ihe nchekwa ndu iji dokwaa pH nke mmeghachi omume biochemical. N'ihi alkalinity ya adịghị ike, a na-ejikarị Tris na-ejikọta ya na acids. A na-ejikarị ya maka nucleic acid, protein dissolution buffer, protein imprinting buffer, DNA imprinting experiment buffer, DNA agarose gel electrophoresis buffer, wdg N'ime usoro mmeghachi omume n'ihi ntinye nke enzymes, protein na ihe mgbochi dị iche iche, ya mere ihe nchekwa ihe chọrọ bụ. dịtụ elu, ya mere ịzụrụ kwesịrị ịhọrọ onye na-emepụta ihe na ọtụtụ afọ nke nyocha na mmepe na mmepụta ahụmahụ, ịdị ọcha dị elu, obere adịghị ọcha iji hụ na ziri ezi nke nnwale biochemical. Mmetụta okpomọkụ Tris buffer, dị mfe ịnweta carbon dioxide na ikuku, nhazi nke ihe nchekwa ahụ ga-ekpuchirịrị nke ọma.

3. Trimethylol aminomethane maka nyocha ọgwụ na mmepe

Atọ hydroxymethyl aminomethane ọzọ utu aha, ya bụ trometamol, dị ka enweghị sodium amino buffer isi, n'ime ahụ mmiri mmiri na-emeghachi omume na H2CO3, belata H2CO3, mepụta HCO32 - ọzọ n'otu oge ahụ, nwere ike banye hydrogen ions na dozie acidemia, mmetụta ya dị ike karị. , na ike site na cell akpụkpọ ahụ, na-ejikarị na nnukwu metabolic na iku ume acidemia; Dị ka ọgwụ alkaline, nke a na-eji maka mgbazi nke acidosis n'emeghị ka njigide carbon dioxide dịkwuo elu. N'ihi na a na-eji ya eme ihe maka ọgwụ, mmepụta dum nke TRIS raw material source, ụzọ usoro mmepụta ihe, ịdị ọcha ngwaahịa, ọdịnaya ion adịghị ọcha bụ ihe ndị a chọrọ, ịzụta ụdị ngwaahịa a ga-abụrịrị nlezianya na nlezianya, ga-agafe ule siri ike.

4. A na-eji Trimethylaminomethane na mmiri nchekwa nje

N'ime ihe nchekwa nchekwa nje, Tris nwere ike buru ụzọ dobe uru pH nke ihe nlele ahụ, na-eme ihe dị ka ihe nchekwa ndu, yana pH buffer nso nke Tris bụ: 7.0 ruo 9.0, ikike iji nọgide na-ewepụ ihe nlele ahụ mgbe ahapụchara ya. nkwụsi ike nke acid nucleic, iji zere mmebi nke nucleic acid, ma melite ntinye na ịdị ọcha nke nucleic acid, na-ekwe nkwa izi ezi nke ọrụ ndị na-esote dị ka nyocha nyocha nke nucleic acid, n'ihi oge ọrịa pụrụ iche, ihe nchọpụta nucleic acid chọrọ, Tris. zuo ibu ga-ibu, ya mere na-atụ aro ịzụta ndị dị otú ahụ ngwaahịa ga-ebu ụzọ zụta a obere ego nke ngwaahịa tupu ịzụrụ nnukwu ikpe kwadoro na ọ dịghị nsogbu, nwekwara ga-enwe teknuzu mgbe-ire-enye ọkachamara oru nkwado.